• Western blot analysis of extracts of various cell lines, using EXOSC1 antibody (STJ112340) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EXOSC1 antibody (1-195) (STJ112340)

SKU:
STJ112340

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EXOSC1 (1-195) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EXOSC1
Gene ID: 51013
Uniprot ID: EXOS1_HUMAN
Immunogen Region: 1-195
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human EXOSC1 (NP_057130.1).
Immunogen Sequence: MAPPVRYCIPGERLCNLEEG SPGSGTYTRHGYIFSSLAGC LMKSSENGALPVVSVVRETE SQLLPDVGAIVTCKVSSINS RFAKVHILYVGSMPLKNSFR GTIRKEDVRATEKDKVEIYK SFRPGDIVLAKVISLGDAQS NYLLTTAENELGVVVAHSES GIQMVPISWCEMQCPKTHTK EFRKVARVQPEFLQT
Function Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC1 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC6 and EXOSC8.
Protein Name Exosome Complex Component Csl4
Exosome Component 1
Database Links Reactome: R-HSA-380994
Reactome: R-HSA-429958
Reactome: R-HSA-450385
Reactome: R-HSA-450513
Reactome: R-HSA-450604
Reactome: R-HSA-6791226
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Alternative Antibody Names Anti-Exosome Complex Component Csl4 antibody
Anti-Exosome Component 1 antibody
Anti-EXOSC1 antibody
Anti-CSL4 antibody
Anti-CGI-108 antibody

Information sourced from Uniprot.org

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