| Host: | Goat |
| Applications: | Pep-ELISA/IF/FC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ETV1 (Internal) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.IF: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10µg/ml. FC:Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | ETV1 |
| Gene ID: | 2115 |
| Uniprot ID: | ETV1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_004947.2; NP_001156619.1; NP_001156620.1; NP_001156621.1; NP_001156622.1; NP_001156623.1; NP_001156624.1 |
| Specificity: | This antidbody is expected to recognise all reported isoforms. Variants (NP_001156620.1; NP_001156621.1) encode the same isoform. |
| Immunogen Sequence: | PLKIKKEPHSPCSE |
| Post Translational Modifications | Sumoylated. Phosphorylated at Ser-191 and Ser-216 by RPS6KA1 and RPS6KA5.phosphorylation activates transcriptional activity. |
| Function | Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGAAT-3'. Required for olfactory dopaminergic neuron differentiation.may directly activate expression of tyrosine hydroxylase (TH). |
| Protein Name | Ets Translocation Variant 1Ets-Related Protein 81 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets Translocation Variant 1 antibodyAnti-Ets-Related Protein 81 antibodyAnti-ETV1 antibodyAnti-ER81 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186370/408075/STJ11107174_1__85519.1744894498.jpg?c=1 "Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.")