| Applications: | IHC-P/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ETV1 |
| Gene ID: | 2115 |
| Uniprot ID: | ETV1_HUMAN |
| Immunogen Region: | 80-300 |
| Immunogen Sequence: | AFHGLPLKIKKEPHSPCSEI SSACSQEQPFKFSYGEKCLY NVSAYDQKPQVGMRPSNPPT PSSTPVSPLHHASPNSTHTP KPDRAFPAHLPPSQSIPDSS YPMDHRFRRQLSEPCNSFPP LPTMPREGRPMYQRQMSEPN IPFPPQGFKQEYHDPVYEHN TMVGSAASQSFPPPLMIKQE PRDFAYDSEVPSCHSIYMRQ EGFLAHPSRTEGCMFEKGPR Q |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 80-300 of human ETV1 (NP_004947.2). |
| Tissue Specificity | Very highly expressed in brain, highly expressed in testis, lung and heart, moderately in spleen, small intestine, pancreas and colon, weakly in liver, prostate and thymus, very weakly in skeletal muscle, kidney and ovary and not in placenta and peripheral blood leukocytes. |
| Post Translational Modifications | Sumoylated. Phosphorylated at Ser-191 and Ser-216 by RPS6KA1 and RPS6KA5.phosphorylation activates transcriptional activity. |
| Function | Transcriptional activator that binds to DNA sequences containing the consensus pentanucleotide 5'-CGGAAT-3'. Required for olfactory dopaminergic neuron differentiation.may directly activate expression of tyrosine hydroxylase (TH). |
| Protein Name | Ets Translocation Variant 1Ets-Related Protein 81 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets Translocation Variant 1 antibodyAnti-Ets-Related Protein 81 antibodyAnti-ETV1 antibodyAnti-ER81 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/186370/408075/STJ11107174_1__85519.1744894498.jpg?c=1 "Western blot analysis of lysates from SH-SY5Y cells using [KD Validated] ETV1 Rabbit mAb (STJ11107174) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 45s.")
