| Host: |
Rabbit |
| Applications: |
WB/IHC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ETFA (1-333) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ETFA |
| Gene ID: |
2108 |
| Uniprot ID: |
ETFA_HUMAN |
| Immunogen Region: |
1-333 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1). |
| Immunogen Sequence: |
MFRAAAPGQLRRAASLLRFQ STLVIAEHANDSLAPITLNT ITAATRLGGEVSCLVAGTKC DKVAQDLCKVAGIAKVLVAQ HDVYKGLLPEELTPLILATQ KQFNYTHICAGASAFGKNLL PRVAAKLEVAPISDIIAIKS PDTFVRTIYAGNALCTVKCD EKVKVFSVRGTSFDAAATSG GSASSEKASSTSPVEISEWL DQKLTKSDRPELTGAKVVVS GGRGLKSGENFKLLYDLAD |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. |
| Protein Name | Electron Transfer Flavoprotein Subunit Alpha - MitochondrialAlpha-Etf |
| Database Links | Reactome: R-HSA-611105 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Electron Transfer Flavoprotein Subunit Alpha - Mitochondrial antibodyAnti-Alpha-Etf antibodyAnti-ETFA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89749/358226/STJ11100813_1__44625.1713122552.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ETFA knockout (KO) 293T cells, using [KO Validated] ETFA Rabbit polyclonal antibody (STJ11100813) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
