![Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89677/358170/STJ11100739_1__87167.1713122476.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89677/358170/STJ11100739_1__87167.1713122476.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
Anti-ESD antibody (1-282) (STJ11100739)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ESD (1-282) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ESD |
| Gene ID | 2098 |
| Uniprot ID | ESTD_HUMAN |
| Immunogen Region | 1-282 |
| Immunogen Sequence | MALKQISSNKCFGGLQKVFE HDSVELNCKMKFAVYLPPKA ETGKCPALYWLSGLTCTEQN FISKSGYHQSASEHGLVVIA PDTSPRGCNIKGEDESWDFG TGAGFYVDATEDPWKTNYRM YSYVTEELPQLINANFPVDP QRMSIFGHSMGGHGALICAL KNPGKYKSVSAFAPICNPVL CPWGKKAFSGYLGTDQSKWK AYDATHLVKSYPGSQLDILI DQGKDDQFLLDGQLLPDNF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human ESD (NP_001975.1). |
Additional Info
| Function | Serine hydrolase involved in the detoxification of formaldehyde. |
| Protein Name | S-Formylglutathione HydrolaseFghEsterase DMethylumbelliferyl-Acetate Deacetylase |
| Database Links | Reactome: R-HSA-156590 |
| Cellular Localisation | CytoplasmCytoplasmic Vesicle |
| Alternative Antibody Names | Anti-S-Formylglutathione Hydrolase antibodyAnti-Fgh antibodyAnti-Esterase D antibodyAnti-Methylumbelliferyl-Acetate Deacetylase antibodyAnti-ESD antibody |
Information sourced from Uniprot.org