| Host: | Goat |
| Applications: | Pep-ELISA/WB-Trf/IHC/IP |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-ERK3/MAPK6 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: In transfected HEK293 transiently expressing Mouse ERK3/Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands a |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | MAPK6 |
| Gene ID: | 5597 |
| Uniprot ID: | MK06_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_002739.1 |
| Specificity: | Reported variants represent identical protein (NP_056621.4; NP_081694.1). |
| Immunogen Sequence: | HSPVGSPLKSIQ |
| Post Translational Modifications | Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Ubiquitination at Met-1 leads to degradation by the proteasome pathway. |
| Function | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle. |
| Protein Name | Mitogen-Activated Protein Kinase 6 Map Kinase 6 Mapk 6 Extracellular Signal-Regulated Kinase 3 Erk-3 Map Kinase Isoform P97 P97-Mapk |
| Database Links | Reactome: R-HSA-5687128 |
| Cellular Localisation | Cytoplasm Nucleus Translocates To The Cytoplasm Following Interaction With Mapkapk5 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 6 antibody Anti-Map Kinase 6 antibody Anti-Mapk 6 antibody Anti-Extracellular Signal-Regulated Kinase 3 antibody Anti-Erk-3 antibody Anti-Map Kinase Isoform P97 antibody Anti-P97-Mapk antibody Anti-MAPK6 antibody Anti-ERK3 antibody Anti-PRKM6 antibody |
Information sourced from Uniprot.org