• Western blot analysis of extracts of various cell lines, using EPS15L1 antibody (STJ110138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EPS15L1 antibody (475-754) (STJ110138)

SKU:
STJ110138

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPS15L1 (475-754) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPS15L1
Gene ID: 58513
Uniprot ID: EP15R_HUMAN
Immunogen Region: 475-754
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 475-754 of human EPS15L1 (NP_001245304.1).
Immunogen Sequence: QMISSLKTQIQSQESDLKSQ EDDLNRAKSELNRLQQEETQ LEQSIQAGRVQLETIIKSLK STQDEINQARSKLSQLHESR QEAHRSLEQYDQVLDGAHGA SLTDLANLSEGVSLAERGSF GAMDDPFKNKALLFSNNTQE LHPDPFQTEDPFKSDPFKGA DPFKGDPFQNDPFAEQQTTS TDPFGGDPFKESDPFRGSAT DDFFKKQTKNDPFTSDPFTK NPSLPSKLDPFESSDPFSS
Post Translational Modifications Phosphorylated on tyrosine residues by EGFR.
Function Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR).internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Protein Name Epidermal Growth Factor Receptor Substrate 15-Like 1
Eps15-Related Protein
Eps15r
Database Links Reactome: R-HSA-182971
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Nucleus
Membrane
Coated Pit
Localized To Plasma Membrane Coated Pits
Alternative Antibody Names Anti-Epidermal Growth Factor Receptor Substrate 15-Like 1 antibody
Anti-Eps15-Related Protein antibody
Anti-Eps15r antibody
Anti-EPS15L1 antibody
Anti-EPS15R antibody

Information sourced from Uniprot.org

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