• Western blot analysis of extracts of various cell lines, using EPS15 antibody (STJ111856) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunofluorescence analysis of C6 cells using EPS15 rabbit polyclonal antibody (STJ111856) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using EPS15 rabbit polyclonal antibody (STJ111856) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using EPS15 rabbit polyclonal antibody (STJ111856) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-EPS15 antibody (660-840) (STJ111856)

SKU:
STJ111856

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPS15 (660-840) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPS15
Gene ID: 2060
Uniprot ID: EPS15_HUMAN
Immunogen Region: 660-840
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 660-840 of human EPS15 (NP_001972.1).
Immunogen Sequence: RQSTDPFATSSTDPFSAANN SSITSVETLKHNDPFAPGGT VVAASDSATDPFASVFGNES FGGGFADFSTLSKVNNEDPF RSATSSSVSNVVITKNVFEE TSVKSEDEPPALPPKIGTPT RPCPLPPGKRSINKLDSPDP FKLNDPFQPFPGNDSPKEKD PEIFCDPFTSATTTTNKEAD P
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Ubiquitinated.
Function Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR).internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.
Protein Name Epidermal Growth Factor Receptor Substrate 15
Protein Eps15
Protein Af-1p
Database Links Reactome: R-HSA-182971
Reactome: R-HSA-6807004
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8875360
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Membrane
Clathrin-Coated Pit
Recruited To The Plasma Membrane Upon Egfr Activation And Localizes To Coated Pits
Colocalizes With Ubqln1 In Ubiquitin-Rich Cytoplasmic Aggregates That Are Not Endocytic Compartments And In Cytoplasmic Juxtanuclear Structures Called Aggresomes
Isoform 2: Early Endosome Membrane
Colocalizes With Hgs On Bilayered Clathrin Coats On Endosomes
Alternative Antibody Names Anti-Epidermal Growth Factor Receptor Substrate 15 antibody
Anti-Protein Eps15 antibody
Anti-Protein Af-1p antibody
Anti-EPS15 antibody
Anti-AF1P antibody

Information sourced from Uniprot.org

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