• Western blot analysis of extracts of various cell lines, using EPHX1 antibody (STJ23551) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-EPHX1 antibody (20-320) (STJ23551)

SKU:
STJ23551

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPHX1 (20-320) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPHX1
Gene ID: 2052
Uniprot ID: HYEP_HUMAN
Immunogen Region: 20-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-320 of human EPHX1 (NP_000111.1).
Immunogen Sequence: SRDKEETLPLEDGWWGPGTR SAAREDDSIRPFKVETSDEE IHDLHQRIDKFRFTPPLEDS CFHYGFNSNYLKKVISYWRN EFDWKKQVEILNRYPHFKTK IEGLDIHFIHVKPPQLPAGH TPKPLLMVHGWPGSFYEFYK IIPLLTDPKNHGLSDEHVFE VICPSIPGYGFSEASSKKGF NSVATARIFYKLMLRLGFQE FYIQGGDWGSLICTNMAQLV PSHVKGLHLNMALVLSNFS
Tissue Specificity Found in liver.
Function Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol.
Protein Name Epoxide Hydrolase 1
Epoxide Hydratase
Microsomal Epoxide Hydrolase
Meh
Database Links Reactome: R-HSA-211945
Cellular Localisation Microsome Membrane
Single-Pass Type Iii Membrane Protein
Endoplasmic Reticulum Membrane
Alternative Antibody Names Anti-Epoxide Hydrolase 1 antibody
Anti-Epoxide Hydratase antibody
Anti-Microsomal Epoxide Hydrolase antibody
Anti-Meh antibody
Anti-EPHX1 antibody
Anti-EPHX antibody
Anti-EPOX antibody

Information sourced from Uniprot.org

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