• Confocal imaging of Rat colon using EpCAM Rabbit monoclonal antibody (STJ11101674, dilution 1:50) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.
  • Confocal imaging of Human colon using EpCAM Rabbit monoclonal antibody (STJ11101674, dilution 1:50) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.
  • Western blot analysis of extracts of various cell lines, using EpCAM Rabbit monoclonal antibody (STJ11101674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-EPCAM antibody (215-314) [S4MR] (STJ11101674)

SKU:
STJ11101674

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-EpCAM (215-314) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPCAM
Gene ID: 4072
Uniprot ID: EPCAM_HUMAN
Immunogen Region: 215-314
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 215-314 of human EpCAM (P16422).
Immunogen Sequence: YFEKDVKGESLFHSKKMDLT VNGEQLDLDPGQTLIYYVDE KAPEFSMQGLKAGVIAVIVV VVIAVVAGIVVLVISRKKRM AKYEKAEIKEMGEMHRELNA
Tissue Specificity Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Post Translational Modifications Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Function May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Protein Name Epithelial Cell Adhesion Molecule
Ep-Cam
Adenocarcinoma-Associated Antigen
Cell Surface Glycoprotein Trop-1
Epithelial Cell Surface Antigen
Epithelial Glycoprotein
Egp
Epithelial Glycoprotein 314
Egp314
Hegp314
Ks 1/4 Antigen
Ksa
Major Gastrointestinal Tumor-Associated Protein Ga733-2
Tumor-Associated Calcium Signal Transducer 1
Cd Antigen Cd326
Database Links Reactome: R-HSA-202733
Cellular Localisation Lateral Cell Membrane
Single-Pass Type I Membrane Protein
Cell Junction
Tight Junction
Colocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction
Alternative Antibody Names Anti-Epithelial Cell Adhesion Molecule antibody
Anti-Ep-Cam antibody
Anti-Adenocarcinoma-Associated Antigen antibody
Anti-Cell Surface Glycoprotein Trop-1 antibody
Anti-Epithelial Cell Surface Antigen antibody
Anti-Epithelial Glycoprotein antibody
Anti-Egp antibody
Anti-Epithelial Glycoprotein 314 antibody
Anti-Egp314 antibody
Anti-Hegp314 antibody
Anti-Ks 1/4 Antigen antibody
Anti-Ksa antibody
Anti-Major Gastrointestinal Tumor-Associated Protein Ga733-2 antibody
Anti-Tumor-Associated Calcium Signal Transducer 1 antibody
Anti-Cd Antigen Cd326 antibody
Anti-EPCAM antibody
Anti-GA733-2 antibody
Anti-M1S2 antibody
Anti-M4S1 antibody
Anti-MIC18 antibody
Anti-TACSTD1 antibody
Anti-TROP1 antibody

Information sourced from Uniprot.org

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