• Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, EPAS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of Jurkat, L929 cells using EPAS-1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
  • Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, EPAS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, EPAS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, EPAS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, EPAS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, EPAS-1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of mouse-spleen tissue. 1, EPAS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of mouse-spleen tissue. 1, EPAS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-stomach tissue. 1, EPAS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-stomach tissue. 1, EPAS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EPAS1 antibody (STJ96690)

SKU:
STJ96690

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IF/WB/IHC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Endothelial PAS domain-containing protein 1 is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-1:2000
IHC-P 1:100-1:300
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPAS1
Gene ID: 2034
Uniprot ID: EPAS1_HUMAN
Specificity: EPAS-1 Polyclonal Antibody detects endogenous levels of EPAS-1 protein.
Immunogen: Synthesized peptide derived from the human EPAS-1 around the non-acetylation site of K385.
Post Translational Modifications In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
Function Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT.heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD.
Protein Name Endothelial Pas Domain-Containing Protein 1
Epas-1
Basic-Helix-Loop-Helix-Pas Protein Mop2
Class E Basic Helix-Loop-Helix Protein 73
Bhlhe73
Hif-1-Alpha-Like Factor
Hlf
Hypoxia-Inducible Factor 2-Alpha
Hif-2-Alpha
Hif2-Alpha
Member Of Pas Protein 2
Pas Domain-Containing Protein 2
Database Links Reactome: R-HSA-1234158
Reactome: R-HSA-1234174
Reactome: R-HSA-1234176
Reactome: R-HSA-452723
Reactome: R-HSA-8849473
Reactome: R-HSA-8951664
Reactome: R-HSA-9664873
Cellular Localisation Nucleus
Nucleus Speckle
Colocalizes With Hif3a In The Nucleus And Speckles
Alternative Antibody Names Anti-Endothelial Pas Domain-Containing Protein 1 antibody
Anti-Epas-1 antibody
Anti-Basic-Helix-Loop-Helix-Pas Protein Mop2 antibody
Anti-Class E Basic Helix-Loop-Helix Protein 73 antibody
Anti-Bhlhe73 antibody
Anti-Hif-1-Alpha-Like Factor antibody
Anti-Hlf antibody
Anti-Hypoxia-Inducible Factor 2-Alpha antibody
Anti-Hif-2-Alpha antibody
Anti-Hif2-Alpha antibody
Anti-Member Of Pas Protein 2 antibody
Anti-Pas Domain-Containing Protein 2 antibody
Anti-EPAS1 antibody
Anti-BHLHE73 antibody
Anti-HIF2A antibody
Anti-MOP2 antibody
Anti-PASD2 antibody

Information sourced from Uniprot.org

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