• Western blot analysis of lysates from THP-1 cells, using EML3 Rabbit polyclonal antibody (STJ11101449) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EML3 antibody (300-530) (STJ11101449)

SKU:
STJ11101449

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EML3 (300-530) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EML3
Gene ID: 256364
Uniprot ID: EMAL3_HUMAN
Immunogen Region: 300-530
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-530 of human EML3 (NP_694997.2).
Immunogen Sequence: HYRGHTDCVRCLAVHPDGVR VASGQTAGVDKDGKPLQPVV HIWDSETLLKLQEIGLGAFE RGVGALAFSAADQGAFLCVV DDSNEHMLSVWDCSRGMKLA EIKSTNDSVLAVGFNPRDSS CIVTSGKSHVHFWNWSGGVG VPGNGTLTRKQGVFGKYKKP KFIPCFVFLPDGDILTGDSE GNILTWGRSPSDSKTPGRGG AKETYGIVAQAHAHEGSIFA LCLRRDGTVLS
Post Translational Modifications Phosphorylation at Thr-881 during mitosis is required for interaction with TUBG1, HAUS1, HAUS2, HAUS3, HAUS4, HAUS5, HAUS6, HAUS7 and HAUS8 and their recruitment to spindle microtubules.
Function Regulates mitotic spindle assembly, microtubule (MT)-kinetochore attachment and chromosome separation via recruitment of HAUS augmin-like complex and TUBG1 to the existing MTs and promoting MT-based MT nucleation. Required for proper alignnment of chromosomes during metaphase.
Protein Name Echinoderm Microtubule-Associated Protein-Like 3
Emap-3
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Midbody
Spindle
Localizes To Microtubules Throughout All Mitotic Stages And Localizes To The Midbody During Cytokinesis
Alternative Antibody Names Anti-Echinoderm Microtubule-Associated Protein-Like 3 antibody
Anti-Emap-3 antibody
Anti-EML3 antibody

Information sourced from Uniprot.org

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