| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EML3 (300-530) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EML3 |
| Gene ID: |
256364 |
| Uniprot ID: |
EMAL3_HUMAN |
| Immunogen Region: |
300-530 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 300-530 of human EML3 (NP_694997.2). |
| Immunogen Sequence: |
HYRGHTDCVRCLAVHPDGVR VASGQTAGVDKDGKPLQPVV HIWDSETLLKLQEIGLGAFE RGVGALAFSAADQGAFLCVV DDSNEHMLSVWDCSRGMKLA EIKSTNDSVLAVGFNPRDSS CIVTSGKSHVHFWNWSGGVG VPGNGTLTRKQGVFGKYKKP KFIPCFVFLPDGDILTGDSE GNILTWGRSPSDSKTPGRGG AKETYGIVAQAHAHEGSIFA LCLRRDGTVLS |
| Post Translational Modifications | Phosphorylation at Thr-881 during mitosis is required for interaction with TUBG1, HAUS1, HAUS2, HAUS3, HAUS4, HAUS5, HAUS6, HAUS7 and HAUS8 and their recruitment to spindle microtubules. |
| Function | Regulates mitotic spindle assembly, microtubule (MT)-kinetochore attachment and chromosome separation via recruitment of HAUS augmin-like complex and TUBG1 to the existing MTs and promoting MT-based MT nucleation. Required for proper alignnment of chromosomes during metaphase. |
| Protein Name | Echinoderm Microtubule-Associated Protein-Like 3Emap-3 |
| Cellular Localisation | CytoplasmCytoskeletonNucleusMidbodySpindleLocalizes To Microtubules Throughout All Mitotic Stages And Localizes To The Midbody During Cytokinesis |
| Alternative Antibody Names | Anti-Echinoderm Microtubule-Associated Protein-Like 3 antibodyAnti-Emap-3 antibodyAnti-EML3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
