| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RBM15 (530-780) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RBM15 |
| Gene ID: |
64783 |
| Uniprot ID: |
RBM15_HUMAN |
| Immunogen Region: |
530-780 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 530-780 of human RBM15 (NP_001188474.1). |
| Immunogen Sequence: |
EHRYQQQYLQPLPLTHYELV TDAFGHRAPDPLRGARDRTP PLLYRDRDRDLYPDSDWVPP PPPVRERSTRTAATSVPAYE PLDSLDRRRDGWSLDRDRGD RDLPSSRDQPRKRRLPEESG GRHLDRSPESDRPRKRHCAP SPDRSPELSSSRDRYNSDND RSSRLLLERPSPIRDRRGSL EKSQGDKRDRKNSASAERDR KHRTTAPTEGKSPLKKEDRS DGSAPSTSTASSKLKSPSQ |
| Post Translational Modifications | Methylated at Arg-578 by PRMT1, leading to promote ubiquitination by CNOT4 and subsequent degradation by the proteasome. Ubiquitinated by CNOT4 following methylation at Arg-578 by PRMT1. |
| Function | RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA. Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex. Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist. Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation. Regulates megakaryocyte differentiation by regulating alternative splicing of genes important for megakaryocyte differentiation.probably regulates alternative splicing via m6A regulation. Required for placental vascular branching morphogenesis and embryonic development of the heart and spleen. Acts as a regulator of thrombopoietin response in hematopoietic stem cells by regulating alternative splicing of MPL. May also function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing. High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing. May be implicated in HOX gene regulation. |
| Protein Name | Rna-Binding Protein 15One-Twenty Two Protein 1Rna-Binding Motif Protein 15 |
| Cellular Localisation | Nucleus SpeckleNucleusNucleoplasmNucleus EnvelopeNucleus MembranePeripheral Membrane ProteinColocalizes At The Nuclear Pore With Dbp5 And Nxf1 |
| Alternative Antibody Names | Anti-Rna-Binding Protein 15 antibodyAnti-One-Twenty Two Protein 1 antibodyAnti-Rna-Binding Motif Protein 15 antibodyAnti-RBM15 antibodyAnti-OTT antibodyAnti-OTT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
