| Short Description : | Rabbit polyclonal EME1 (1-320) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EME1 |
| Gene ID: | 146956 |
| Uniprot ID: | EME1_HUMAN |
| Immunogen Region: | 1-320 |
| Immunogen Sequence: | MALKKSSPSLDSGDSDSEEL PTFAFLKKEPSSTKRRQPER EEKIVVVDISDCEASCPPAP ELFSPPVPEIAETVTQTQPV RLLSSESEDEEEFIPLAQRL TCKFLTHKQLSPEDSSSPVK SVLDHQNNEGASCDWKKPFP KIPEVPLHDTPERSAADNKD LILDPCCQLPAYLSTCPGQS SSLAVTKTNSDILPPQKKTK PSQKVQGRGSHGCRQQRQAR QKESTLRRQERKNAALVTR |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EME1 (NP_689676.2). |
| Function | Non-catalytic subunit of the structure-specific, heterodimeric DNA endonuclease MUS81-EME1 which is involved in the maintenance of genome stability. In the complex, EME1 is required for DNA cleavage, participating in DNA recognition and bending. MUS81-EME1 cleaves 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs. Active during prometaphase, MUS81-EME1 resolves mitotic recombination intermediates, including Holliday junctions, which form during homologous recombination. |
| Protein Name | Structure-Specific Endonuclease Subunit Eme1Crossover Junction Endonuclease Eme1Essential Meiotic Structure-Specific Endonuclease 1Mms4 HomologHmms4 |
| Database Links | Reactome: R-HSA-5693568Reactome: R-HSA-6783310 |
| Cellular Localisation | NucleusNucleolusRecruited To Regions Of Dna Damage In S-Phase Cells |
| Alternative Antibody Names | Anti-Structure-Specific Endonuclease Subunit Eme1 antibodyAnti-Crossover Junction Endonuclease Eme1 antibodyAnti-Essential Meiotic Structure-Specific Endonuclease 1 antibodyAnti-Mms4 Homolog antibodyAnti-Hmms4 antibodyAnti-EME1 antibodyAnti-MMS4 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of HeLa cells using [KO Validated] Annexin A5/Annexin V Rabbit polyclonal antibody (STJ22620) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/99134/383749/STJ22620_1__87132.1713152313.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] Annexin A5/Annexin V Rabbit polyclonal antibody (STJ22620) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
