Anti-EIF3G antibody (1-320) (STJ23512)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EIF3G (1-320) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF3G |
| Gene ID | 8666 |
| Uniprot ID | EIF3G_HUMAN |
| Immunogen Region | 1-320 |
| Immunogen Sequence | MPTGDFDSKPSWADQVEEEG EDDKCVTSELLKGIPLATGD TSPEPELLPGAPLPPPKEVI NGNIKTVTEYKIDEDGKKFK IVRTFRIETRKASKAVARRK NWKKFGNSEFDPPGPNVATT TVSDDVSMTFITSKEDLNCQ EEEDPMNKLKGQKIVSCRIC KGDHWTTRCPYKDTLGPMQK ELAEQLGLSTGEKEKLPGEL EPVQATQNKTGKYVPPSLRD GASRRGESMQPNRRADDNA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EIF3G (NP_003746.2). |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation is enhanced upon serum stimulation. |
| Function | RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit GEif3gEukaryotic Translation Initiation Factor 3 Rna-Binding SubunitEif-3 Rna-Binding SubunitEukaryotic Translation Initiation Factor 3 Subunit 4Eif-3-DeltaEif3 P42Eif3 P44 |
| Database Links | Reactome: R-HSA-156827Reactome: R-HSA-72649Reactome: R-HSA-72689Reactome: R-HSA-72695Reactome: R-HSA-72702Reactome: R-HSA-72706 |
| Cellular Localisation | CytoplasmNucleusPerinuclear RegionColocalizes With Aifm1 In The Nucleus And Perinuclear Region |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 3 Subunit G antibodyAnti-Eif3g antibodyAnti-Eukaryotic Translation Initiation Factor 3 Rna-Binding Subunit antibodyAnti-Eif-3 Rna-Binding Subunit antibodyAnti-Eukaryotic Translation Initiation Factor 3 Subunit 4 antibodyAnti-Eif-3-Delta antibodyAnti-Eif3 P42 antibodyAnti-Eif3 P44 antibodyAnti-EIF3G antibodyAnti-EIF3S4 antibody |
Information sourced from Uniprot.org