• Immunohistochemistry analysis of paraffin-embedded human appendix using ELMO1 Rabbit monoclonal antibody (STJ11102280) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using ELMO1 Rabbit monoclonal antibody (STJ11102280) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ELMO1 Rabbit monoclonal antibody (STJ11102280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ELMO1 antibody (628-727) [S0MR] (STJ11102280)

SKU:
STJ11102280

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ELMO1 (628-727) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELMO1
Gene ID: 9844
Uniprot ID: ELMO1_HUMAN
Immunogen Region: 628-727
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 628-727 of human ELMO1 (Q92556).
Immunogen Sequence: KGALKQNKEVLELAFSILYD SNCQLNFIAPDKHEYCIWTD GLNALLGKDMMSDLTRNDLD TLLSMEIKLRLLDLENIQIP DAPPPIPKEPSNYDFVYDCN
Tissue Specificity Widely expressed, with a higher expression in the spleen and placenta.
Post Translational Modifications Phosphorylated by HCK.
Function Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Protein Name Engulfment And Cell Motility Protein 1
Protein Ced-12 Homolog
Database Links Reactome: R-HSA-164944
Reactome: R-HSA-2029482
Reactome: R-HSA-4420097
Reactome: R-HSA-8849471
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cell Membrane
Translocation To Plasma Membrane Seems To Be Mediated By Dock1 And Crk
Alternative Antibody Names Anti-Engulfment And Cell Motility Protein 1 antibody
Anti-Protein Ced-12 Homolog antibody
Anti-ELMO1 antibody
Anti-KIAA0281 antibody

Information sourced from Uniprot.org

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