• Immunofluorescence analysis of A549 cells using ELF5 antibody (STJ29261). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using ELF5 Rabbit polyclonal antibody (STJ29261) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using ELF5 Rabbit polyclonal antibody (STJ29261) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ELF5 antibody (STJ29261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ELF5 antibody (40-150) (STJ29261)

SKU:
STJ29261

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ELF5 (40-150) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELF5
Gene ID: 2001
Uniprot ID: ELF5_HUMAN
Immunogen Region: 40-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-150 of human ELF5 (NP_938195.1).
Immunogen Sequence: EEYYPAFEHQTACDSYWTSV HPEYWTKRHVWEWLQFCCDQ YKLDTNCISFCNFNISGLQL CSMTQEEFVEAAGLCGEYLY FILQNIRTQGYSFFNDAEES KATIKDYADSN
Tissue Specificity Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher. Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression.
Function Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGAAT. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters.
Protein Name Ets-Related Transcription Factor Elf-5
E74-Like Factor 5
Epithelium-Restricted Ese-1-Related Ets Factor
Epithelium-Specific Ets Transcription Factor 2
Ese-2
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ets-Related Transcription Factor Elf-5 antibody
Anti-E74-Like Factor 5 antibody
Anti-Epithelium-Restricted Ese-1-Related Ets Factor antibody
Anti-Epithelium-Specific Ets Transcription Factor 2 antibody
Anti-Ese-2 antibody
Anti-ELF5 antibody
Anti-ESE2 antibody

Information sourced from Uniprot.org

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