• Western blot analysis of the lysates from K562 cells using KLF antibody.
  • Western blot analysis of VEC cells using EKLF/CKLF/UKLF Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of lysates from Jurkat cells, treated with serum 20% 15', using KLF Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using EKLF/CKLF/UKLF Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-EKLF/CKLF/UKLF antibody (291-340 aa) (STJ92887)

SKU:
STJ92887

Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Krueppel-like factor 1 and Krueppel-like factor 5 and Krueppel-like factor 7 (291-340 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLF7
KLF1
8609
10661
688
KLF7_HUMAN
KLF1_HUMAN
KLF5_HUMAN
Immunogen Region: 291-340 aa
Specificity: EKLF/CKLF/UKLF Polyclonal Antibody detects endogenous levels of EKLF/CKLF/UKLF protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human KLF at the amino acid range 291-340
Post Translational Modifications Acetylated.can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity. Sumoylated.sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex. Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.
Function Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
Protein Name Krueppel-Like Factor 1
Erythroid Krueppel-Like Transcription Factor
Eklf
Cellular Localisation Nucleus
Colocalizes With Sumo1 In Nuclear Speckles
Alternative Antibody Names Anti-Krueppel-Like Factor 1 antibody
Anti-Erythroid Krueppel-Like Transcription Factor antibody
Anti-Eklf antibody
Anti-KLF1 antibody
Anti-EKLF antibody

Information sourced from Uniprot.org

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