| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EIF6 (1-245) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EIF6 |
| Gene ID: |
3692 |
| Uniprot ID: |
IF6_HUMAN |
| Immunogen Region: |
1-245 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6 (NP_852133.1). |
| Immunogen Sequence: |
MAVRASFENNCEIGCFAKLT NTYCLVAIGGSENFYSVFEG ELSDTIPVVHASIAGCRIIG RMCVGNRHGLLVPNNTTDQE LQHIRNSLPDTVQIRRVEER LSALGNVTTCNDYVALVHPD LDRETEEILADVLKVEVFRQ TVADQVLVGSYCVFSNQGGL VHPKTSIEDQDELSSLLQVP LVAGTVNRGSEVIAAGMVVN DWCAFCGLDTTSTELSVVES VFKLNEAQPSTIATSMRDS |
| Tissue Specificity | Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). |
| Post Translational Modifications | Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export. Ufmylated by UFL1. |
| Function | Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis. Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth. |
| Protein Name | Eukaryotic Translation Initiation Factor 6Eif-6B(2gcn HomologB4 Integrin InteractorCabP27(Bbp |
| Cellular Localisation | CytoplasmNucleusNucleolusShuttles Between Cytoplasm And Nucleus/Nucleolus |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 6 antibodyAnti-Eif-6 antibodyAnti-B(2gcn Homolog antibodyAnti-B4 Integrin Interactor antibodyAnti-Cab antibodyAnti-P27(Bbp antibodyAnti-EIF6 antibodyAnti-EIF3A antibodyAnti-ITGB4BP antibodyAnti-OK antibodyAnti-SW-cl.27 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
