Anti-EIF5B antibody (1051-1100 aa) (STJ92886)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human EIF5B at the amino acid range 1051-1100
General Information
| Short Description | Rabbit polyclonal anti-Eukaryotic translation initiation factor 5B (1051-1100 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EIF5B |
| Gene ID | 9669 |
| Uniprot ID | IF2P_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human EIF5B at the amino acid range 1051-1100 |
| Immunogen Region | 1051-1100 aa |
| Specificity | eIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein. |
Additional Info
| Function | Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit. |
| Protein Name | Eukaryotic Translation Initiation Factor 5bEif-5bTranslation Initiation Factor If-2 |
| Database Links | Reactome: R-HSA-72706 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 5b antibodyAnti-Eif-5b antibodyAnti-Translation Initiation Factor If-2 antibodyAnti-EIF5B antibodyAnti-IF2 antibodyAnti-KIAA0741 antibody |
Information sourced from Uniprot.org