| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Eukaryotic translation initiation factor 3 subunit E is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EIF3E |
| Gene ID: | 3646 |
| Uniprot ID: | EIF3E_HUMAN |
| Specificity: | This antibody detects endogenous levels of EIF3E at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human EIF3E |
| Function | Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Required for nonsense-mediated mRNA decay (NMD).may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. |
| Protein Name | Eukaryotic Translation Initiation Factor 3 Subunit E Eif3e Eukaryotic Translation Initiation Factor 3 Subunit 6 Viral Integration Site Protein Int-6 Homolog Eif-3 P48 |
| Database Links | Reactome: R-HSA-156827 Reactome: R-HSA-72649 Reactome: R-HSA-72689 Reactome: R-HSA-72695 Reactome: R-HSA-72702 Reactome: R-HSA-72706 |
| Cellular Localisation | Cytoplasm Nucleus Pml Body |
| Alternative Antibody Names | Anti-Eukaryotic Translation Initiation Factor 3 Subunit E antibody Anti-Eif3e antibody Anti-Eukaryotic Translation Initiation Factor 3 Subunit 6 antibody Anti-Viral Integration Site Protein Int-6 Homolog antibody Anti-Eif-3 P48 antibody Anti-EIF3E antibody Anti-EIF3S6 antibody Anti-INT6 antibody |
Information sourced from Uniprot.org