• Immunohistochemistry analysis of paraffin-embedded rat brain using EIF2S3 antibody (STJ28664) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using EIF2S3 antibody (STJ28664) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using EIF2S3 antibody (STJ28664) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using EIF2S3 antibody (STJ28664) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2S3 antibody (1-250) (STJ28664)

SKU:
STJ28664

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EIF2S3 (1-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2S3
Gene ID: 1968
Uniprot ID: IF2G_HUMAN
Immunogen Region: 1-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EIF2S3 (NP_001406.1).
Immunogen Sequence: MAGGEAGVTLGQPHLSRQDL TTLDVTKLTPLSHEVISRQA TINIGTIGHVAHGKSTVVKA ISGVHTVRFKNELERNITIK LGYANAKIYKLDDPSCPRPE CYRSCGSSTPDEFPTDIPGT KGNFKLVRHVSFVDCPGHDI LMATMLNGAAVMDAALLLIA GNESCPQPQTSEHLAAIEIM KLKHILILQNKIDLVKESQA KEQYEQILAFVQGTVAEGAP IIPISAQLKYNIEVVCEYI
Tissue Specificity Expressed in testis, brain, liver and muscle.
Function Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form the 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Protein Name Eukaryotic Translation Initiation Factor 2 Subunit 3
Eukaryotic Translation Initiation Factor 2 Subunit Gamma X
Eif-2-Gamma X
Eif-2gx
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-381042
Reactome: R-HSA-382556
Reactome: R-HSA-72649
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72731
Reactome: R-HSA-9633012
Reactome: R-HSA-9648895
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 2 Subunit 3 antibody
Anti-Eukaryotic Translation Initiation Factor 2 Subunit Gamma X antibody
Anti-Eif-2-Gamma X antibody
Anti-Eif-2gx antibody
Anti-EIF2S3 antibody
Anti-EIF2G antibody

Information sourced from Uniprot.org

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