• Immunofluorescence analysis of NIH/3T3 cells using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of EIF2B2 in paraffin-embedded mouse brain using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EIF2B2 in paraffin-embedded human tonsil using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of EIF2B2 in paraffin-embedded rat lung using EIF2B2 Rabbit polyclonal antibody (STJ29107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using EIF2B2 Rabbit polyclonal antibody (STJ29107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF2B2 antibody (1-120) (STJ29107)

SKU:
STJ29107

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2B2
Gene ID: 8892
Uniprot ID: EI2BB_HUMAN
Immunogen Region: 1-120
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human EIF2B2 (NP_055054.1).
Immunogen Sequence: MPGSAAKGSELSERIESFVE TLKRGGGPRSSEEMARETLG LLRQIITDHRWSNAGELMEL IRREGRRMTAAQPSETTVGN MVRRVLKIIREEYGRLHGRS DESDQQESLHKLLTSGGLNE
Function Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.
Protein Name Translation Initiation Factor Eif2b Subunit Beta
S20i15
S20iii15
Eif2b Gdp-Gtp Exchange Factor Subunit Beta
Database Links Reactome: R-HSA-72731
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Translation Initiation Factor Eif2b Subunit Beta antibody
Anti-S20i15 antibody
Anti-S20iii15 antibody
Anti-Eif2b Gdp-Gtp Exchange Factor Subunit Beta antibody
Anti-EIF2B2 antibody
Anti-EIF2BB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance