• Western blot analysis using EHMT1 monoclonal antibody against EHMT1 recombinant protein.

Anti-EHMT1 antibody [1B7B4] (STJ98015)

SKU:
STJ98015

Current Stock:
Host: Mouse
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Histone-lysine N-methyltransferase EHMT1 is suitable for use in Western Blot and ELISA research applications.
Clonality: Monoclonal
Clone ID: 1B7B4
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide.
Purification: Affinity purification
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EHMT1
Gene ID: 79813
Uniprot ID: EHMT1_HUMAN
Specificity: EHMT1 Monoclonal Antibody detects endogenous levels of EHMT1 protein.
Immunogen: Purified recombinant fragment of EHMT1 expressed in E. Coli.
Function Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Represses the expression of mitochondrial function-related genes, perhaps by occupying their promoter regions, working in concert with probable chromatin reader BAZ2B.
Protein Name Histone-Lysine N-Methyltransferase Ehmt1
Euchromatic Histone-Lysine N-Methyltransferase 1
Eu-Hmtase1
G9a-Like Protein 1
Glp
Glp1
Histone H3-K9 Methyltransferase 5
H3-K9-Hmtase 5
Lysine N-Methyltransferase 1d
Database Links Reactome: R-HSA-2559582
Reactome: R-HSA-3214841
Reactome: R-HSA-6804760
Reactome: R-HSA-8853884
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Chromosome
Associates With Euchromatic Regions
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Ehmt1 antibody
Anti-Euchromatic Histone-Lysine N-Methyltransferase 1 antibody
Anti-Eu-Hmtase1 antibody
Anti-G9a-Like Protein 1 antibody
Anti-Glp antibody
Anti-Glp1 antibody
Anti-Histone H3-K9 Methyltransferase 5 antibody
Anti-H3-K9-Hmtase 5 antibody
Anti-Lysine N-Methyltransferase 1d antibody
Anti-EHMT1 antibody
Anti-EUHMTASE1 antibody
Anti-GLP antibody
Anti-KIAA1876 antibody
Anti-KMT1D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance