Anti-EHHADH antibody (476-525 aa) (STJ94975)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human EHHADH at the amino acid range 476-525
General Information
| Short Description | Rabbit polyclonal anti-Peroxisomal bifunctional enzyme (476-525 aa) for use in WB, IHC, IF and ELISA in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EHHADH |
| Gene ID | 1962 |
| Uniprot ID | ECHP_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human EHHADH at the amino acid range 476-525 |
| Immunogen Region | 476-525 aa |
| Specificity | PBFE Polyclonal Antibody detects endogenous levels of PBFE protein. |
Additional Info
| Post Translational Modifications | Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. |
| Function | Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway. Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity. Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways. Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation. |
| Protein Name | Peroxisomal Bifunctional EnzymePbePbfeL-Bifunctional ProteinLbpMultifunctional Enzyme 1Mfe1 Includes - Enoyl-Coa Hydratase/3 -2-Trans-Enoyl-Coa Isomerase - 3-Hydroxyacyl-Coa Dehydrogenase |
| Database Links | Reactome: R-HSA-390247Reactome: R-HSA-9033241 |
| Cellular Localisation | Peroxisome |
| Alternative Antibody Names | Anti-Peroxisomal Bifunctional Enzyme antibodyAnti-Pbe antibodyAnti-Pbfe antibodyAnti-L-Bifunctional Protein antibodyAnti-Lbp antibodyAnti-Multifunctional Enzyme 1 antibodyAnti-Mfe1 Includes - Enoyl-Coa Hydratase/3 -2-Trans-Enoyl-Coa Isomerase - 3-Hydroxyacyl-Coa Dehydrogenase antibodyAnti-EHHADH antibodyAnti-ECHD antibody |
Information sourced from Uniprot.org