• Western blot analysis of extracts of various cell lines, using eEF2 rabbit monoclonal antibody (STJ11102532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using eEF2 Rabbit monoclonal antibody (STJ11102532) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat spleen, using eEF2 rabbit monoclonal antibody (STJ11102532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of NIH-3T3 cells using eEF2 Rabbit monoclonal antibody (STJ11102532) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human appendix using eEF2 rabbit monoclonal antibody (STJ11102532) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using eEF2 Rabbit monoclonal antibody (STJ11102532) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using eEF2 rabbit monoclonal antibody (STJ11102532) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of eEF2 in paraffin-embedded human appendix using eEF2 Rabbit monoclonal antibody (STJ11102532) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using eEF2 rabbit monoclonal antibody (STJ11102532) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat spleen, using eEF2 Rabbit monoclonal antibody (STJ11102532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U-2 OS cells using eEF2 rabbit monoclonal antibody (STJ11102532) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using eEF2 Rabbit monoclonal antibody (STJ11102532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-EEF2 antibody (759-858) [S2MR] (STJ11102532)

SKU:
STJ11102532

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-EEF2 (759-858) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF2
Gene ID: 1938
Uniprot ID: EF2_HUMAN
Immunogen Region: 759-858
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 759-858 of human eEF2 (P13639).
Immunogen Sequence: IYGVLNRKRGHVFEESQVAG TPMFVVKAYLPVNESFGFTA DLRSNTGGQAFPQCVFDHWQ ILPGDPFDNSSRPSQVVAET RKRKGLKEGIPALDNFLDKL
Post Translational Modifications Phosphorylation by EF-2 kinase completely inactivates EF-2.it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase. Phosphorylation by CSK promotes SUMOylation, proteolytic cleavage, and nuclear translocation if the C-terminal fragment. Diphthamide is 2-3-carboxyamido-3-(trimethyl-ammonio)propylhistidine. (Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis. ISGylated. Proteolytically processed at two sites following phosphorylation by CSK. SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage.
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Protein Name Elongation Factor 2
Ef-2
Database Links Reactome: R-HSA-156902
Reactome: R-HSA-5336415
Reactome: R-HSA-5358493
Reactome: R-HSA-6798695
Reactome: R-HSA-8876725
Cellular Localisation Cytoplasm
Nucleus
Phosphorylation By Csk Promotes Cleavage And Sumoylation-Dependent Nuclear Translocation Of The C-Terminal Cleavage Product
Alternative Antibody Names Anti-Elongation Factor 2 antibody
Anti-Ef-2 antibody
Anti-EEF2 antibody
Anti-EF2 antibody

Information sourced from Uniprot.org

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