• Immunoprecipitation of EEF1D in 300 µg extracts from Jurkat cells using 3 µg EEF1D Rabbit polyclonal antibody (STJ23477). Western blot analysis was performed using EEF1D Rabbit polyclonal antibody (STJ23477) at 1:500 dilution.
  • Immunoprecipitation of EEF1D in 300 µg extracts from jurkat cells using 3 µg EEF1D Rabbit polyclonal antibody (STJ23477). Western blot analysis was performed using EEF1D Rabbit polyclonal antibody (STJ23477) at 1:500 dilution.
  • Immunofluorescence analysis of PC-12 cells using EEF1D Rabbit polyclonal antibody (STJ23477) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using EEF1D Rabbit polyclonal antibody (STJ23477) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of EEF1D in paraffin-embedded Rat kidney tissue using EEF1D Rabbit polyclonal antibody (STJ23477) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EEF1D in paraffin-embedded Mouse brain tissue using EEF1D Rabbit polyclonal antibody (STJ23477) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EEF1D in paraffin-embedded Human kidney tissue using EEF1D Rabbit polyclonal antibody (STJ23477) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of EEF1D in paraffin-embedded Human liver tissue using EEF1D Rabbit polyclonal antibody (STJ23477) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using EEF1D Rabbit polyclonal antibody (STJ23477) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EEF1D antibody (1-160) (STJ23477)

SKU:
STJ23477

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EEF1D (1-160) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF1D
Gene ID: 1936
Uniprot ID: EF1D_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).
Immunogen Sequence: MATNFLAHEKIWFDKFKYDD AERRFYEQMNGPVAGASRQE NGASVILRDIARARENIQKS LAGSSGPGASSGTSGDHGEL VVRIASLEVENQSLRGVVQE LQQAISKLEARLNVLEKSSP GHRATAPQTQHVSPMRQVEP PAKKPATPAEDDEDDDIDLF
Tissue Specificity Isoform 2 is specifically expressed in brain, cerebellum and testis.
Function Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome. Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
Protein Name Elongation Factor 1-Delta
Ef-1-Delta
Antigen Ny-Co-4
Database Links Reactome: R-HSA-156842
Cellular Localisation Isoform 2: Nucleus
Alternative Antibody Names Anti-Elongation Factor 1-Delta antibody
Anti-Ef-1-Delta antibody
Anti-Antigen Ny-Co-4 antibody
Anti-EEF1D antibody
Anti-EF1D antibody

Information sourced from Uniprot.org

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