• Endogenous EEA1 detected with antibody at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunostaining of Hepa1-6 cells with EEA1 antibody at 1:50 dilution

Anti-EEA1 antibody (1230aa C-Term) (STJ140033)

SKU:
STJ140033

£100.50 - £393.50
Current Stock:
Host: Goat
Applications: WB/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Early Endosome antigen 1 (1230aa C-Term) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:250-1:1000
IF 1:50-1:250
Beck MCG PhD Thesis University of Ulm Germany 2018
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: EEA1
Gene ID: 8411
Uniprot ID: EEA1_HUMAN
Immunogen Region: 1230aa C-Term
Accession Number: ENSG00000102189
Specificity: Detects a band of 180 kDa by Western blot in the following human, mouse and rat whole cell lysates.
Immunogen: Purified recombinant peptide within residues 1, 230 aa to the C-terminus of human EEA1 produced in E. coli.
Immunogen Sequence: KKHEENEAKLTMQITALNEN LGTVKKEWQSSQRRVSELEK QTDDLRGEIAVLEATVQNNQ DERRALLERCLKGEGEIEKL QTKVLELQRKLDNTTAAVQE LGRENQSLQIKHTQALNRKW AEDNEVQNCMACGKGFSVTV RRHHCRQCGNIFCAECSAKN ALTPSSKKPVRVCDACFNDL QG
Function Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Protein Name Early Endosome Antigen 1
Endosome-Associated Protein P162
Zinc Finger Fyve Domain-Containing Protein 2
Database Links Reactome: R-HSA-168138
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Early Endosome Antigen 1 antibody
Anti-Endosome-Associated Protein P162 antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 2 antibody
Anti-EEA1 antibody
Anti-ZFYVE2 antibody

Information sourced from Uniprot.org

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