Host: | Rabbit |
Applications: | WB/IHC-P/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | EDF1 |
Gene ID: | 8721 |
Uniprot ID: | EDF1_HUMAN |
Immunogen Region: | 1-148 |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human EDF1 (NP_003783.1). |
Immunogen Sequence: | MAESDWDTVTVLRKKGPTAA QAKSKQAILAAQRRGEDVET SKKWAAGQNKQHSITKNTAK LDRETEELHHDRVTLEVGKV IQQGRQSKGLTQKDLATKIN EKPQVIADYESGRAIPNNQV LGKIERAIGLKLRGKDIGKP IEKGPRAK |
Tissue Specificity | Expressed in brain, liver, lung, kidney and heart (at protein level). Ubiquitously expressed. More abundant in heart, pancreas, liver, intestine and adipose tissues. |
Post Translational Modifications | Phosphorylated (by PKA and PKC). |
Function | Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism. |
Protein Name | Endothelial Differentiation-Related Factor 1Edf-1Multiprotein-Bridging Factor 1Mbf1 |
Cellular Localisation | CytoplasmNucleusAlso Nuclear Upon Binding To Nr5a1 And Treatment Of Cells With Tpa Or Forskolin |
Alternative Antibody Names | Anti-Endothelial Differentiation-Related Factor 1 antibodyAnti-Edf-1 antibodyAnti-Multiprotein-Bridging Factor 1 antibodyAnti-Mbf1 antibodyAnti-EDF1 antibody |
Information sourced from Uniprot.org