• Western blot analysis of extracts of HepG2 cells, using EDA antibody (STJ23467) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human uterine cancer using EDA Antibody (STJ23467) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using EDA Antibody (STJ23467) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using EDA Antibody (STJ23467) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using EDA rabbit polyclonal antibody (STJ23467) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using EDA rabbit polyclonal antibody (STJ23467) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-251MG cells using EDA rabbit polyclonal antibody (STJ23467) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-EDA antibody (62-182) (STJ23467)

SKU:
STJ23467

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EDA (62-182) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EDA
Gene ID: 1896
Uniprot ID: EDA_HUMAN
Immunogen Region: 62-182
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 62-182 of human EDA (NP_001390.1).
Immunogen Sequence: LELRSELRRERGAESRLGGS GTPGTSGTLSSLGGLDPDSP ITSHLGQPSPKQQPLEPGEA ALHSDSQDGHQMALLNFFFP DEKPYSEEESRRVRRNKRSK SNEGADGPVKNKKKGKKAGP P
Tissue Specificity Not abundant.expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord.
Post Translational Modifications N-glycosylated. Processing by furin produces a secreted form.
Function Cytokine which is involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Functions as a ligand activating the DEATH-domain containing receptors EDAR and EDA2R. May also play a role in cell adhesion. Isoform 1: Binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor EDA2R. Isoform 3: Binds only to the receptor EDA2R.
Protein Name Ectodysplasin-A
Ectodermal Dysplasia Protein
Eda Protein Cleaved Into - Ectodysplasin-A - Membrane Form - Ectodysplasin-A - Secreted Form
Database Links Reactome: R-HSA-5669034 Q92838-3
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Ectodysplasin-A
Secreted Form: Secreted
Alternative Antibody Names Anti-Ectodysplasin-A antibody
Anti-Ectodermal Dysplasia Protein antibody
Anti-Eda Protein Cleaved Into - Ectodysplasin-A - Membrane Form - Ectodysplasin-A - Secreted Form antibody
Anti-EDA antibody
Anti-ED1 antibody
Anti-EDA2 antibody

Information sourced from Uniprot.org

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