Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-ECM1 antibody (N-Term) (STJ92820)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the N-terminal region of human ECM1.
STJ92820
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General Information

Short DescriptionRabbit polyclonal anti-Extracellular matrix protein 1 (N-Term) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/IF/ELISA
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeIHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolECM1
Gene ID1893
Uniprot IDECM1_HUMAN
ImmunogenSynthesized peptide derived from the N-terminal region of human ECM1.
Immunogen RegionN-Term
SpecificityECM1 Polyclonal Antibody detects endogenous levels of ECM1 protein.

Additional Info

Function Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.
Protein Name Extracellular Matrix Protein 1
Secretory Component P85
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Extracellular Matrix Protein 1 antibody
Anti-Secretory Component P85 antibody
Anti-ECM1 antibody

Information sourced from Uniprot.org

Citations

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