• Immunofluorescence analysis of A431 cells using ECM1 Rabbit polyclonal antibody (STJ118796) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A375 cells using ECM1 Rabbit polyclonal antibody (STJ118796) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ECM1 Rabbit polyclonal antibody (STJ118796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ECM1 antibody (280-405) (STJ118796)

SKU:
STJ118796

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECM1 (280-405) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECM1
Gene ID: 1893
Uniprot ID: ECM1_HUMAN
Immunogen Region: 280-405
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 280-405 of human ECM1 (NP_004416.2).
Immunogen Sequence: QLRACPSHQPDISSGLELPF PPGVPTLDNIKNICHLRRFR SVPRNLPATDPLQRELLALI QLEREFQRCCRQGNNHTCTW KAWEDTLDKYCDREYAVKTH HHLCCRHPPSPTRDECFARR APYPNY
Tissue Specificity Expressed in breast cancer tissues. Little or no expression observed in normal breast tissues. Expressed in skin.wide expression is observed throughout the dermis with minimal expression in the epidermis.
Function Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.
Protein Name Extracellular Matrix Protein 1
Secretory Component P85
Database Links Reactome: R-HSA-114608
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Extracellular Matrix Protein 1 antibody
Anti-Secretory Component P85 antibody
Anti-ECM1 antibody

Information sourced from Uniprot.org

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