• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using E2F4 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from COS7 cells, using E2F4 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using E2F-4/5 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-E2F-4/5 antibody (51-100 aa) (STJ92809)

SKU:
STJ92809

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcription factor E2F4 and Transcription factor E2F5 (51-100 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: E2F5
E2F4
Gene ID: 1875
1874
Uniprot ID: E2F5_HUMAN
E2F4_HUMAN
Immunogen Region: 51-100 aa
Specificity: E2F-4/5 Polyclonal Antibody detects endogenous levels of E2F-4/5 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human E2F4 at the amino acid range 51-100
Post Translational Modifications Differentially phosphorylated in vivo.
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Protein Name Transcription Factor E2f4
E2f-4
Database Links Reactome: R-HSA-1362277
Reactome: R-HSA-1362300
Reactome: R-HSA-1538133
Reactome: R-HSA-2173796
Reactome: R-HSA-6804114
Reactome: R-HSA-69202
Reactome: R-HSA-69205
Reactome: R-HSA-69231
Reactome: R-HSA-69656
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor E2f4 antibody
Anti-E2f-4 antibody
Anti-E2F4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance