| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Cadherin-1 and Cadherin-2 and Cadherin-3 and Cadherin-4 (833-882 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC-P 1:100-300 ELISA 1:20000 IF 1:100-300 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CDH4 CDH1 1002 999 1001 CADH4_HUMAN CADH1_HUMAN CADH3_HUMAN |
| Immunogen Region: | 833-882 aa |
| Specificity: | E-cadherin Polyclonal Antibody detects endogenous levels of E-cadherin protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Cadherin at the amino acid range 833-882 |
| Post Translational Modifications | During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation. N-glycosylation at Asn-637 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-754. O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Thr-285 and Thr-509 are O-mannosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes.internalin A (InlA) binds to this protein and promotes uptake of the bacteria. |
| Protein Name | Cadherin-1 Cam 120/80 Epithelial Cadherin E-Cadherin Uvomorulin Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 |
| Database Links | Reactome: R-HSA-1474228 Reactome: R-HSA-198933 Reactome: R-HSA-216083 Reactome: R-HSA-351906 Reactome: R-HSA-418990 Reactome: R-HSA-5626467 Reactome: R-HSA-8876493 |
| Cellular Localisation | Cell Junction Adherens Junction Cell Membrane Single-Pass Type I Membrane Protein Endosome Golgi Apparatus Trans-Golgi Network Colocalizes With Dlgap5 At Sites Of Cell-Cell Contact In Intestinal Epithelial Cells Anchored To Actin Microfilaments Through Association With Alpha- Beta- And Gamma-Catenin Sequential Proteolysis Induced By Apoptosis Or Calcium Influx Results In Translocation From Sites Of Cell-Cell Contact To The Cytoplasm Colocalizes With Rab11a Endosomes During Its Transport From The Golgi Apparatus To The Plasma Membrane |
| Alternative Antibody Names | Anti-Cadherin-1 antibody Anti-Cam 120/80 antibody Anti-Epithelial Cadherin antibody Anti-E-Cadherin antibody Anti-Uvomorulin antibody Anti-Cd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 antibody Anti-CDH1 antibody Anti-CDHE antibody Anti-UVO antibody |
Information sourced from Uniprot.org