| Short Description : | Goat polyclonal antibody anti-dUTPase/DUT (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications: | Pep-ELISA/WB/IHC |
| Host: | Goat |
| Reactivity: | Human/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 20kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 17.7kDa according to NP_001939.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DUT |
| Gene ID: | 1854 |
| Uniprot ID: | DUT_HUMAN |
| Accession Number: | NP_001020419.1; NP_001939.1; NP_001020420.1 |
| Immunogen Region: | Internal |
| Immunogen Sequence: | QLRFARLSEHATAPT |
| Specificity: | This antibody is expected to recognize all reported isoforms (NP_001020419.1; NP_001939.1; NP_001020420.1). |
| Post Translational Modifications | Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated. |
| Function | Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development. |
| Protein Name | Deoxyuridine 5'-Triphosphate Nucleotidohydrolase - MitochondrialDutpaseDutp Pyrophosphatase |
| Database Links | Reactome: R-HSA-499943 P33316-2 |
| Cellular Localisation | Isoform 2: NucleusIsoform 3: Mitochondrion |
| Alternative Antibody Names | Anti-Deoxyuridine 5'-Triphosphate Nucleotidohydrolase - Mitochondrial antibodyAnti-Dutpase antibodyAnti-Dutp Pyrophosphatase antibodyAnti-DUT antibody |
Information sourced from Uniprot.org
