• Immunofluorescence analysis of U-2 OS cells using DUT Rabbit polyclonal antibody (STJ117799) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DUT in paraffin-embedded human appendix using DUT Rabbit polyclonal antibody (STJ117799) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DUT in paraffin-embedded human liver cancer using DUT Rabbit polyclonal antibody (STJ117799) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DUT in paraffin-embedded rat spleen using DUT Rabbit polyclonal antibody (STJ117799) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using DUT Rabbit polyclonal antibody (STJ117799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-DUT antibody (89-252) (STJ117799)

SKU:
STJ117799

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DUT (89-252) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DUT
Gene ID: 1854
Uniprot ID: DUT_HUMAN
Immunogen Region: 89-252
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 89-252 of human DUT (NP_001020419.1).
Immunogen Sequence: PAPGPETPAISPSKRARPAE VGGMQLRFARLSEHATAPTR GSARAAGYDLYSAYDYTIPP MEKAVVKTDIQIALPSGCYG RVAPRSGLAAKHFIDVGAGV IDEDYRGNVGVVLFNFGKEK FEVKKGDRIAQLICERIFYP EIEEVQALDDTERGSGGFGS TGKN
Tissue Specificity Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level).
Post Translational Modifications Nuclear isoform 2 is phosphorylated in vivo on Ser-11, a reaction that can be catalyzed in vitro by CDC2. Phosphorylation in mature T-cells occurs in a cell cycle-dependent manner. Isoform 3 is not phosphorylated.
Function Catalyzes the cleavage of 2'-deoxyuridine 5'-triphosphate (dUTP) into 2'-deoxyuridine 5'-monophosphate (dUMP) and inorganic pyrophosphate and through its action efficiently prevents uracil misincorporation into DNA and at the same time provides dUMP, the substrate for de novo thymidylate biosynthesis. Inhibits peroxisome proliferator-activated receptor (PPAR) activity by binding of its N-terminal to PPAR, preventing the latter's dimerization with retinoid X receptor. Essential for embryonic development.
Protein Name Deoxyuridine 5'-Triphosphate Nucleotidohydrolase - Mitochondrial
Dutpase
Dutp Pyrophosphatase
Database Links Reactome: R-HSA-499943 P33316-2
Cellular Localisation Isoform 2: Nucleus
Isoform 3: Mitochondrion
Alternative Antibody Names Anti-Deoxyuridine 5'-Triphosphate Nucleotidohydrolase - Mitochondrial antibody
Anti-Dutpase antibody
Anti-Dutp Pyrophosphatase antibody
Anti-DUT antibody

Information sourced from Uniprot.org

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