• Western blot analysis of Rat lung, using DUSP8 rabbit polyclonal antibody (STJ119964) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of HeLa cells using DUSP8 rabbit polyclonal antibody (STJ119964) at dilution of 1:1000 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using DUSP8 rabbit polyclonal antibody (STJ119964) at dilution of 1:1000 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using DUSP8 rabbit polyclonal antibody (STJ119964) at dilution of 1:1000 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using DUSP8 rabbit polyclonal antibody (STJ119964) at dilution of 1:1000 (40x lens). Blue: DAPI for nuclear staining.

Anti-DUSP8 antibody (1-14) (STJ119964)

SKU:
STJ119964

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DUSP8 (1-14) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DUSP8
Gene ID: 1850
Uniprot ID: DUS8_HUMAN
Immunogen Region: 1-14
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-14 of human DUSP8 (NP_004411.2).
Immunogen Sequence: NleAGDRLPRKVNl
Tissue Specificity Abundant in brain, heart and skeletal muscle.
Function Has phosphatase activity with synthetic phosphatase substrates and negatively regulates mitogen-activated protein kinase activity, presumably by catalysing their dephosphorylation. Expected to display protein phosphatase activity toward phosphotyrosine, phosphoserine and phosphothreonine residues.
Protein Name Dual Specificity Protein Phosphatase 8
Dual Specificity Protein Phosphatase Hvh-5
Database Links Reactome: R-HSA-112409
Reactome: R-HSA-5675221
Reactome: R-HSA-9652817
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Dual Specificity Protein Phosphatase 8 antibody
Anti-Dual Specificity Protein Phosphatase Hvh-5 antibody
Anti-DUSP8 antibody
Anti-C11orf81 antibody
Anti-VH5 antibody

Information sourced from Uniprot.org

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