• Western blot analysis of extracts of various cell lines, using DUSP16 antibody (STJ112194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DUSP16 antibody (416-665) (STJ112194)

SKU:
STJ112194

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DUSP16 (416-665) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DUSP16
Gene ID: 80824
Uniprot ID: DUS16_HUMAN
Immunogen Region: 416-665
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 416-665 of human DUSP16 (NP_085143.1).
Immunogen Sequence: SLHGFSSSEDALEYYKPSTT LDGTNKLCQFSPVQELSEQT PETSPDKEEASIPKKLQTAR PSDSQSKRLHSVRTSSSGTA QRSLLSPLHRSGSVEDNYHT SFLFGLSTSQQHLTKSAGLG LKGWHSDILAPQTSTPSLTS SWYFATESSHFYSASAIYGG SASYSAYSCSQLPTCGDQVY SVRRRQKPSDRADSRRSWHE ESPFEKQFKRRSCQMEFGES IMSENRSREELGKVGSQSS
Post Translational Modifications Phosphorylated at Ser-446 by MAPK1/ERK2, which prevents its degradation, and thereby stabilizes it and blocks JNK MAPK activity. (Microbial infection) Acetylated at Lys-55 by the M.tuberculosis Eis protein.this leads to the inhibition of JNK-dependent autophagy, phagosome maturation, and ROS (reactive oxygen species) generation for enhanced intracellular survival of M.tuberculosis.
Function Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.
Protein Name Dual Specificity Protein Phosphatase 16
Mitogen-Activated Protein Kinase Phosphatase 7
Map Kinase Phosphatase 7
Mkp-7
Database Links Reactome: R-HSA-112409
Reactome: R-HSA-5675221
Reactome: R-HSA-9636569
Reactome: R-HSA-9652817
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic Vesicle
After Dissociation Upon Agtr Stimulation
Re-Associates With Arrb2 On Endocytic Vesicles
Alternative Antibody Names Anti-Dual Specificity Protein Phosphatase 16 antibody
Anti-Mitogen-Activated Protein Kinase Phosphatase 7 antibody
Anti-Map Kinase Phosphatase 7 antibody
Anti-Mkp-7 antibody
Anti-DUSP16 antibody
Anti-KIAA1700 antibody
Anti-MKP7 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance