Anti-Doublecortin-Mouse antibody (Internal) (STJ72841)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Doublecortin-Mouse (Internal) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Applications | Pep-ELISA/WB/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 40kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to NP_001103692.1). Recommended concentration: 0.5-2ยตg/ml. Primary incubation was 1 hour. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | DCX |
| Gene ID | 1641 |
| Uniprot ID | DCX_HUMAN |
| Accession Number | NP_001103692.1; NP_001103694.1; NP_034155.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | PKASPTPQKTSAKS |
| Specificity | This antibody is expected to recognize all reported mouse isoforms (NP_001103692.1; NP_001103694.1; NP_034155.2). Reported variants represent identical protein: NP_001103692.1, NP_001103693.1 |
Additional Info
| Post Translational Modifications | Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules. Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21. Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells. |
| Function | Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. |
| Protein Name | Neuronal Migration Protein DoublecortinDoublinLissencephalin-XLis-X |
| Database Links | Reactome: R-HSA-447043 |
| Cellular Localisation | CytoplasmCell ProjectionNeuron ProjectionLocalizes At Neurite Tips |
| Alternative Antibody Names | Anti-Neuronal Migration Protein Doublecortin antibodyAnti-Doublin antibodyAnti-Lissencephalin-X antibodyAnti-Lis-X antibodyAnti-DCX antibodyAnti-DBCN antibodyAnti-LISX antibody |
Information sourced from Uniprot.org