| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-DOK3 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 50kDa band observed in Human Bone marrow and Human Peripheral Blood Mononucleocytes lysates (calculated MW of 50.3kDa according to NP_079148.2). Recommended concentration: 2-6µ |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DOK3 |
| Gene ID: | 79930 |
| Uniprot ID: | DOK3_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_079148.2 |
| Immunogen Sequence: | TRERRKGPAPCDRP |
| Post Translational Modifications | Constitutively tyrosine-phosphorylated. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase. |
| Function | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function. |
| Protein Name | Docking Protein 3 Downstream Of Tyrosine Kinase 3 |
| Database Links | Reactome: R-HSA-6798695 |
| Cellular Localisation | Cytoplasm Cell Membrane Peripheral Membrane Protein Cytoplasmic Side |
| Alternative Antibody Names | Anti-Docking Protein 3 antibody Anti-Downstream Of Tyrosine Kinase 3 antibody Anti-DOK3 antibody |
Information sourced from Uniprot.org
