STJ70073 (2µg/ml) staining of Human Bone Marrow (A) and PBL (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DOK3 antibody (C-Term) (STJ70073)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70073-100
🚚 Free UK Delivery on orders over £150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionGoat polyclonal antibody anti-DOK3 (C-Term) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Mouse/Rat/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 50kDa band observed in Human Bone marrow and Human Peripheral Blood Mononucleocytes lysates (calculated MW of 50.3kDa according to NP_079148.2). Recommended concentration: 2-6µ
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolDOK3
Gene ID79930
Uniprot IDDOK3_HUMAN
Accession NumberNP_079148.2
Immunogen RegionC-Term
Immunogen SequenceTRERRKGPAPCDRP

Additional Info

Post Translational Modifications Constitutively tyrosine-phosphorylated. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase.
Function DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function.
Protein Name Docking Protein 3
Downstream Of Tyrosine Kinase 3
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Docking Protein 3 antibody
Anti-Downstream Of Tyrosine Kinase 3 antibody
Anti-DOK3 antibody

Information sourced from Uniprot.org

Citations

Product Review

!