• STJ70073 (2µg/ml) staining of Human Bone Marrow (A) and PBL (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-DOK3 antibody (C-Term) (STJ70073)

SKU:
STJ70073-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-DOK3 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 50kDa band observed in Human Bone marrow and Human Peripheral Blood Mononucleocytes lysates (calculated MW of 50.3kDa according to NP_079148.2). Recommended concentration: 2-6µ
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: DOK3
Gene ID: 79930
Uniprot ID: DOK3_HUMAN
Immunogen Region: C-Term
Accession Number: NP_079148.2
Immunogen Sequence: TRERRKGPAPCDRP
Post Translational Modifications Constitutively tyrosine-phosphorylated. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase.
Function DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function.
Protein Name Docking Protein 3
Downstream Of Tyrosine Kinase 3
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Docking Protein 3 antibody
Anti-Downstream Of Tyrosine Kinase 3 antibody
Anti-DOK3 antibody

Information sourced from Uniprot.org

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