Anti-DOCK8 antibody [R05-7D3] (STJA0034255)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human DOCK8
General Information
| Short Description | Rabbit monoclonal anti-DOCK8 for use in WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R05-7D3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | DOCK8 |
| Gene ID | 81704 |
| Uniprot ID | DOCK8_HUMAN |
| Immunogen | Recombinant protein of human DOCK8 |
Additional Info
| Tissue Specificity | Expressed in peripheral blood mononuclear cells (PBMCs). |
| Post Translational Modifications | In response to chemokine CXCL12/SDF-1-alpha stimulation, phosphorylated by PRKCA/PKC-alpha which promotes DOCK8 dissociation from LRCH1. |
| Function | Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP. During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC. Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane. Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing. |
| Protein Name | Dedicator Of Cytokinesis Protein 8 |
| Database Links | Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013409Reactome: R-HSA-983231 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinCytoplasmic SideCell ProjectionLamellipodium MembraneEnriched And Co-Localizes With Gtpase Cdc42 At The Immunological Synapse Formed During T Cell/Antigen Presenting Cell Cognate InteractionTranslocates From The Cytoplasm To The Plasma Membrane In Response To Chemokine Cxcl12/Sdf-1-Alpha Stimulation |
| Alternative Antibody Names | Anti-Dedicator Of Cytokinesis Protein 8 antibodyAnti-DOCK8 antibody |
Information sourced from Uniprot.org