Confocal imaging of MCF7 cells using DNPH1 Rabbit monoclonal antibody (STJ11103484, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-DNPH1 antibody (75-174) [S4MR] (STJ11103484)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103484
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General Information

Short DescriptionRabbit monoclonal DNPH1 (75-174) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS4MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolDNPH1
Gene ID10591
Uniprot IDDNPH1_HUMAN
Immunogen Region75-174
Immunogen SequenceLIHEQDLEWLQQADVVVAEV TQPSLGVGYELGRAVAFNKR ILCLFRPQSGRVLSAMIRGA ADGSRFQVWDYEEGEVEALL DRYFEADPPGQVAASPDPTT
SpecificityA synthetic peptide corresponding to a sequence within amino acids 75-174 of human DNPH1 (O43598).

Additional Info

Tissue Specificity Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers.
Function Part of a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) in a two-step process entailing deamination to cytotoxic 5-hydroxymethyl-dUMP (hmdUMP), followed by its hydrolysis into 5-hydroxymethyluracil (hmU) and 2-deoxy-D-ribose 5-phosphate (deoxyribosephosphate). Catalyzes the second step in that pathway, the hydrolysis of the N-glycosidic bond in hmdUMP, degrading this cytotoxic nucleotide to avoid its genomic integration.
Protein Name 5-Hydroxymethyl-Dump N-Hydrolase
2'-Deoxynucleoside 5'-Phosphate N-Hydrolase 1
C-Myc-Responsive Protein Rcl
Database Links Reactome: R-HSA-74259
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-5-Hydroxymethyl-Dump N-Hydrolase antibody
Anti-2'-Deoxynucleoside 5'-Phosphate N-Hydrolase 1 antibody
Anti-C-Myc-Responsive Protein Rcl antibody
Anti-DNPH1 antibody
Anti-C6orf108 antibody
Anti-RCL antibody

Information sourced from Uniprot.org

Citations

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