• Confocal imaging of MCF7 cells using DNPH1 Rabbit monoclonal antibody (STJ11103484, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using DNPH1 Rabbit monoclonal antibody (STJ11103484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DNPH1 antibody (75-174) [S4MR] (STJ11103484)

SKU:
STJ11103484

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DNPH1 (75-174) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNPH1
Gene ID: 10591
Uniprot ID: DNPH1_HUMAN
Immunogen Region: 75-174
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 75-174 of human DNPH1 (O43598).
Immunogen Sequence: LIHEQDLEWLQQADVVVAEV TQPSLGVGYELGRAVAFNKR ILCLFRPQSGRVLSAMIRGA ADGSRFQVWDYEEGEVEALL DRYFEADPPGQVAASPDPTT
Tissue Specificity Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers.
Function Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.
Protein Name 2'-Deoxynucleoside 5'-Phosphate N-Hydrolase 1
C-Myc-Responsive Protein Rcl
Database Links Reactome: R-HSA-74259
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-2'-Deoxynucleoside 5'-Phosphate N-Hydrolase 1 antibody
Anti-C-Myc-Responsive Protein Rcl antibody
Anti-DNPH1 antibody
Anti-C6orf108 antibody
Anti-RCL antibody

Information sourced from Uniprot.org

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