• Immunofluorescence analysis of HeLa cells using DNMT3B Rabbit polyclonal antibody (STJ23415) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using DNMT3B Rabbit polyclonal antibody (STJ23415) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using DNMT3B Rabbit polyclonal antibody (STJ23415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-DNMT3B antibody (345-853) (STJ23415)

SKU:
STJ23415

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNMT3B (345-853) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNMT3B
Gene ID: 1789
Uniprot ID: DNM3B_HUMAN
Immunogen Region: 345-853
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 345-853 of human DNMT3B (NP_008823.1).
Immunogen Sequence: IEGLKPNNTQPVVNKSKVRR AGSRKLESRKYENKTRRRTA DDSATSDYCPAPKRLKTNCY NNGKDRGDEDQSREQMASDV ANNKSSLEDGCLSCGRKNPV SFHPLFEGGLCQTCRDRFLE LFYMYDDDGYQ
Tissue Specificity Ubiquitous.highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.
Post Translational Modifications Sumoylated. Citrullinated by PADI4.
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells.
Protein Name Dna
Cytosine-5-Methyltransferase 3b
Dnmt3b
Dna Methyltransferase Hsaiiib
Dna Mtase Hsaiiib
M.hsaiiib
Database Links Reactome: R-HSA-212300
Reactome: R-HSA-427413
Reactome: R-HSA-4655427
Reactome: R-HSA-5334118
Reactome: R-HSA-9710421
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Dna antibody
Anti-Cytosine-5-Methyltransferase 3b antibody
Anti-Dnmt3b antibody
Anti-Dna Methyltransferase Hsaiiib antibody
Anti-Dna Mtase Hsaiiib antibody
Anti-M.hsaiiib antibody
Anti-DNMT3B antibody

Information sourced from Uniprot.org

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