• Immunofluorescence analysis of NIH-3T3 cells using DNAJC15 Rabbit monoclonal antibody (STJ11102161) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DNAJC15 Rabbit monoclonal antibody (STJ11102161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DNAJC15 antibody (70-150) [S1MR] (STJ11102161)

SKU:
STJ11102161

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DNAJC15 (70-150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DNAJC15
Gene ID: 29103
Uniprot ID: DJC15_HUMAN
Immunogen Region: 70-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 70-150 of human DNAJC15 (Q9Y5T4).
Immunogen Sequence: ETAKKISTPSFSSYYKGGFE QKMSRREAGLILGVSPSAGK AKIRTAHRRVMILNHPDKGG SPYVAAKINEAKDLLETTTK H
Tissue Specificity Expressed at highest levels in heart, followed by liver and kidney.
Function Negative regulator of the mitochondrial respiratory chain. Prevents mitochondrial hyperpolarization state and restricts mitochondrial generation of ATP. Acts as an import component of the TIM23 translocase complex. Stimulates the ATPase activity of HSPA9.
Protein Name Dnaj Homolog Subfamily C Member 15
Cell Growth-Inhibiting Gene 22 Protein
Methylation-Controlled J Protein
Mcj
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Dnaj Homolog Subfamily C Member 15 antibody
Anti-Cell Growth-Inhibiting Gene 22 Protein antibody
Anti-Methylation-Controlled J Protein antibody
Anti-Mcj antibody
Anti-DNAJC15 antibody
Anti-DNAJD1 antibody
Anti-GIG22 antibody
Anti-HSD18 antibody

Information sourced from Uniprot.org

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