| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DnaJ homolog subfamily A member 1 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DNAJA1 |
| Gene ID: |
3301 |
| Uniprot ID: |
DNJA1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of DNAJA1 at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human DNAJA1 |
| Function | Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV. |
| Protein Name | Dnaj Homolog Subfamily A Member 1Dnaj Protein Homolog 2HsdjHeat Shock 40 Kda Protein 4Heat Shock Protein J2Hsj-2Human Dnaj Protein 2Hdj-2 |
| Database Links | Reactome: R-HSA-3371497 |
| Cellular Localisation | MembraneLipid-AnchorCytoplasmMicrosomeNucleusPerinuclear RegionMitochondrionPrimarily Associated With MicrosomesA Minor Proportion Is Associated With MitochondriaPrimarily CytoplasmicA Minor Proportion Is Associated With Nuclei |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily A Member 1 antibodyAnti-Dnaj Protein Homolog 2 antibodyAnti-Hsdj antibodyAnti-Heat Shock 40 Kda Protein 4 antibodyAnti-Heat Shock Protein J2 antibodyAnti-Hsj-2 antibodyAnti-Human Dnaj Protein 2 antibodyAnti-Hdj-2 antibodyAnti-DNAJA1 antibodyAnti-DNAJ2 antibodyAnti-HDJ2 antibodyAnti-HSJ2 antibodyAnti-HSPF4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ11100741) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89679/358172/STJ11100741_1__59623.1713122479.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNAJA1 knockout (KO) 293T cells, using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ11100741) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ113207) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/92785/373370/STJ113207_1__86638.1713139598.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ113207) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
