• Immunofluorescence analysis of U-2 OS cells using DLST Rabbit polyclonal antibody (STJ115261) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of DLST in paraffin-embedded human gastric cancer using DLST Rabbit polyclonal antibody (STJ115261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DLST in paraffin-embedded human esophageal cancer using DLST Rabbit polyclonal antibody (STJ115261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DLST in paraffin-embedded human liver damage using DLST Rabbit polyclonal antibody (STJ115261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using DLST Rabbit polyclonal antibody (STJ115261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DLST antibody (1-290) (STJ115261)

SKU:
STJ115261

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DLST
Gene ID: 1743
Uniprot ID: ODO2_HUMAN
Immunogen Region: 1-290
Specificity: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human DLST (NP_001924.2).
Immunogen Sequence: PAKAKPAEAPAAAAPKAEPT AAAVPPPAAPIPTQMPPVPS PSQPPSGKPVSAVKPTVAPP LAEPGAGKGLRSEHREKMNR MRQRIAQRLKEAQNTCAMLT T
Function Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.
Protein Name Dihydrolipoyllysine-Residue Succinyltransferase Component Of 2-Oxoglutarate Dehydrogenase Complex - Mitochondrial
2-Oxoglutarate Dehydrogenase Complex Component E2
Ogdc-E2
Dihydrolipoamide Succinyltransferase Component Of 2-Oxoglutarate Dehydrogenase Complex
E2k
Database Links Reactome: R-HSA-6783984
Reactome: R-HSA-9853506
Reactome: R-HSA-9857492
Reactome: R-HSA-9858328
Cellular Localisation Mitochondrion Matrix
Nucleus
Mainly Localizes In The Mitochondrion
A Small Fraction Localizes To The Nucleus
Where The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation
Alternative Antibody Names Anti-Dihydrolipoyllysine-Residue Succinyltransferase Component Of 2-Oxoglutarate Dehydrogenase Complex - Mitochondrial antibody
Anti-2-Oxoglutarate Dehydrogenase Complex Component E2 antibody
Anti-Ogdc-E2 antibody
Anti-Dihydrolipoamide Succinyltransferase Component Of 2-Oxoglutarate Dehydrogenase Complex antibody
Anti-E2k antibody
Anti-DLST antibody
Anti-DLTS antibody

Information sourced from Uniprot.org

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