| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S0MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DLD |
| Gene ID: | 1738 |
| Uniprot ID: | DLDH_HUMAN |
| Immunogen Region: | 401-500 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 401-500 of human DLDH/DLD (P09622). |
| Immunogen Sequence: | WVGKSEEQLKEEGIEYKVGK FPFAANSRAKTNADTDGMVK ILGQKSTDRVLGAHILGPGA GEMVNEAALALEYGASCEDI ARVCHAHPTLSEAFREANLA |
| Post Translational Modifications | Tyrosine phosphorylated. |
| Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A. In monomeric form may have additional moonlighting function as serine protease. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. |
| Protein Name | Dihydrolipoyl Dehydrogenase - Mitochondrial Dihydrolipoamide Dehydrogenase Glycine Cleavage System L Protein |
| Database Links | Reactome: R-HSA-204174 Reactome: R-HSA-5362517 Reactome: R-HSA-6783984 Reactome: R-HSA-70895 Reactome: R-HSA-9837999 Reactome: R-HSA-9853506 Reactome: R-HSA-9858328 Reactome: R-HSA-9859138 Reactome: R-HSA-9861559 |
| Cellular Localisation | Mitochondrion Matrix Nucleus Cell Projection Cilium Flagellum Cytoplasmic Vesicle Secretory Vesicle Acrosome Mainly Localizes In The Mitochondrion A Small Fraction Localizes To The Nucleus Where The 2-Oxoglutarate Dehydrogenase Complex Is Required For Histone Succinylation |
| Alternative Antibody Names | Anti-Dihydrolipoyl Dehydrogenase - Mitochondrial antibody Anti-Dihydrolipoamide Dehydrogenase antibody Anti-Glycine Cleavage System L Protein antibody Anti-DLD antibody Anti-GCSL antibody Anti-LAD antibody Anti-PHE3 antibody |
Information sourced from Uniprot.org