• Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23967). Blue: DAPI for nuclear staining.
  • Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R17 antibody (STJ23967).
  • Western blot analysis of various lysates using Symmetric DiMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23967) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Di-Methyl-Histone H3-R17 antibody (Around Arg17) (STJ23967)

SKU:
STJ23967

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Symmetric Histone H3-R17 (Around Arg17) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: Around Arg17
Immunogen: A synthetic symmetric dimethylated peptide around R17 of human histone H3 (NP_003520.1).
Immunogen Sequence: APRKQ
Background The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.

Information sourced from Uniprot.org

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